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Polska Bibliografia Naukowa

Issue 12 (3) 2013 pp. 3–11

Olga V. Mushkina, Natalia S. Gurina, Mira M. Konopleva, Wieslawa Bylka, Irena Matlawska

Activity and total phenolic content of Alnus glutinosa and Alnus incana leaves

Abstract: Alnus glutinosa and A. incana (Betulaceae), are a small to medium size tree, native to the Northern Hemisphere. The leaves of this species are used in the Republic of Belarus as a source of antioxidants. The aim of this work was investigation of antioxidant activities and total phenolics content in various extracts obtained using water, mixture water with ethanol (from 9:1 to 2:8), and ethanol from A. glutinosa and A. incana leaves. Phenolics content was determined by method with Folin-Ciocalteu reagent and calculated on ellagic acid. The antioxidant activities were measured utilising 1,1-diphenyl-2 picrylhydrazyl (DPPH) radical scavenging test. The highest phenolics concentration was observed in the extracts prepared by extraction with the mixture of water:ethanol from 7:3 to 3:7 and ranged from 17.82% to 18.96% for A. glutinosa and from 10.82% to 12.55% for A. incana. This extracts exhibited the highest free radical scavenging activity ranging from 49.21% to 49.42% and from 41.28% to 41.67% for A. glutinosa and A. incana respectively, comparable to the activity of quercetin. Therefore the mixture of water:ethanol from 7:3 to 3:7 should be used for preparing extracts from this species for medicinal purposes. Results also indicated the existence of a high correlation between antioxidant activity and total phenolics content.
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Keywords: leaf extracts, DPPH, hiperoside, phenilic acids

For citation:

MLA Mushkina, Olga V., et al. "Activity and total phenolic content of Alnus glutinosa and Alnus incana leaves." Acta Sci.Pol. Hortorum Cultus 12.3 (2013): 12.
APA Mushkina, O. V., Gurina, N. S., Konopleva, M. M., Bylka, W., & Matlawska, I. (2013). Activity and total phenolic content of Alnus glutinosa and Alnus incana leaves. Acta Sci.Pol. Hortorum Cultus 12 (3), 12.
ISO 690 MUSHKINA, Olga V., et al. Activity and total phenolic content of Alnus glutinosa and Alnus incana leaves. Acta Sci.Pol. Hortorum Cultus, 2013, 12.3: 12.
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Streszczenie w języku polskim:
http://www.hortorumcultus.actapol.net/tom12/zeszyt3/abstrakt-3.html