University of Life Sciences in Lublin

Wydawnictwo Uniwersytetu Przyrodniczego

Scopus

Index Copernicus Journal Master List

Polska Bibliografia Naukowa

Issue 13 (1) 2014 pp. 117–127

Ewa Hanus-Fajerska, Alina Wiszniewska, Andrew Riseman

Elaboration of in vitro root culture protocols to efficiently limit Daphne Sudden Death Syndrome

Abstract: In this study in vitro root culture protocol was elaborated in order to diversify screening methods and develop quick and reliable assay to test the level of chosen Daphne genotype resistance to fungal pathogen Thielaviopsis basicola. Plantlets of Daphne caucasica, D. cneorum, D. jasminea, and D. pontica were propagated aseptically on medium composed of WPM mineral salts and MS vitamins, supplemented with 1.0 mg dm-3 2iP, 0.1 mg dm-3 NAA, 0.5 g dm-3 PVP, 0.5 g dm-3 MES, 0.65 g dm-3 calcium gluconate, and 20 g dm-3 sucrose. Root cultures were initiated from adventitious roots regenerated on micropropagated shoots. Proliferative root cultures of examined Daphne species were obtained both on solidified and liquid medium, supplemented with various doses of NAA. Cultures of detached roots proved to be a convenient system of testing to T. basicola resistance. Daphne roots cultured in vitro were distinctly contaminated under laboratory conditions. Susceptibility of cultured organs differed between species. This simple method is appropriate to be put into practice for quick selection of resistant/tolerant genotypes to the soil-borne fungal pathogens affecting plants via root system.
pub/13_1_117.pdf Full text available in Adobe Acrobat format:
http://www.hortorumcultus.actapol.net/volume13/issue1/13_1_117.pdf
Keywords: biotic stress, fungal pathogen, in vitro root culture, Thielaviopsis basicola, DSDS

For citation:

MLA Hanus-Fajerska, Ewa, et al. "Elaboration of in vitro root culture protocols to efficiently limit Daphne Sudden Death Syndrome." Acta Sci.Pol. Hortorum Cultus 13.1 (2014): 13.
APA Hanus-Fajerska, E., Wiszniewska, A., & Riseman, A. (2014). Elaboration of in vitro root culture protocols to efficiently limit Daphne Sudden Death Syndrome. Acta Sci.Pol. Hortorum Cultus 13 (1), 13.
ISO 690 HANUS-FAJERSKA, Ewa, WISZNIEWSKA, Alina, RISEMAN, Andrew. Elaboration of in vitro root culture protocols to efficiently limit Daphne Sudden Death Syndrome. Acta Sci.Pol. Hortorum Cultus, 2014, 13.1: 13.
EndNote BibTeX RefMan
Streszczenie w języku polskim:
http://www.hortorumcultus.actapol.net/tom13/zeszyt1/abstrakt-117.html