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Polska Bibliografia Naukowa

Issue 13 (2) 2014 pp. 197–205

Ahmad Baker, Behzad Kaviani, Ghorbanali Nematzadeh, Naser Negahdar

Micropropagation of Orchis catasetum – a rare and endangered orchid

Abstract: The application of modern biotechnology for mass propagation of rare and endangered species needs to develop a proper in vitro protocol. Here, a protocol was developed for high frequency in vitro multiplication of an endangered orchid, Orchis catasetum. Protocorms, as explants were cultured on Murashige and Skoog (MS) medium fortified with different concentrations of N6-benzyladenine (BA), α-naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA) either individually or in combination. A combination of 0.5 mg·L BA and 0.5 mg·L NAA was found to be suitable for maximum protocorm-like bodies (PLBs) regeneration (20.40 × plantlet-1). The largest number of root (7.16 × plantlet-1) and leaf (10.10 × plantlet-1), also the highest plant height (114.20 mm × plantlet-1) and root length (193.40 mm × plantlet-1) were obtained on MS medium supplemented with 0.5 mg·L BA a long with 0.5 mg · L NAA. Plantlets with well-developed leaves and roots were transplanted to pots filled with perlite, wood pieces, ionolite and mineral cartridge shell (1:1:1:1), also perlite individually and transferred to the greenhouse. Upon ex vitro transfer, 100% of plants survived.
pub/13_2_197.pdf Full text available in Adobe Acrobat format:
http://www.hortorumcultus.actapol.net/volume13/issue2/13_2_197.pdf
Keywords: auxins, cytokinins, endangered species, in vitro propagation, protocorm like bodies (PLBs), tissue culture

For citation:

MLA Baker, Ahmad, et al. "Micropropagation of Orchis catasetum – a rare and endangered orchid." Acta Sci.Pol. Hortorum Cultus 13.2 (2014): 13.
APA Baker, A., Kaviani, B., Nematzadeh, G., & Negahdar, N. (2014). Micropropagation of Orchis catasetum – a rare and endangered orchid. Acta Sci.Pol. Hortorum Cultus 13 (2), 13.
ISO 690 BAKER, Ahmad, et al. Micropropagation of Orchis catasetum – a rare and endangered orchid. Acta Sci.Pol. Hortorum Cultus, 2014, 13.2: 13.
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Streszczenie w języku polskim:
http://www.hortorumcultus.actapol.net/tom13/zeszyt2/abstrakt-197.html