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Issue 13 (2) 2014 pp. 45–58

Alicja Tymoszuk, Małgorzata Zalewska

In vitro adventitious shoots regeneration from ligulate florets in the aspect of application in chrysanthemum breeding

Abstract: Chrysanthemum mutants can be chimeras. The regeneration in vitro of adventitious shoots from ligulate florets can lead to the separation of chimera components, resulting in producing a new cultivar. There was determined the effect of various factors on the number and length of shoots regenerating in vitro from ligulate florets of Chrysanthemum × grandiflorum (Ramat.) Kitam. ‘Cool Time’. The ligulate florets were inoculated on the MS [1962] medium supplemented with cytokinin (0; 4.44; 8.88; 13.32; 22.20 µM∙dm-3 BAP; 4.65; 23.23; 46.47; 69.70 µM∙dm-3 KIN) and auxin (0; 0.54; 1.08; 1.61; 2.69 µM∙dm-3 NAA). Most shoots regenerate when 8.88; 13.32 µM∙dm-3 BAP or 69.70 µM∙dm-3 KIN and 2.69 µ M∙dm-3 NAA or 8.88 µM∙dm-3 BAP and 1.61 µM∙dm-3 NAA are supplemented. Adding 0.29; 1.44 or 2.89 µM∙dm-3 GA3 to the MS medium with 8.88 µM∙dm-3 BAP and 2.69 µM∙dm-3 NAA limits the shoot regeneration efficiency and does not stimulate their elongation. An increase in the shoot number and length is affected by the subculture of regenerating ligulate florets from the MS medium containing 8.88 µM∙dm-3 BAP and 2.69 µ M∙dm-3 NAA on the medium with 2.89 µM∙dm-3 GA3 and 2.69 µM∙dm-3 NAA. There were found no differences in the number and length of shoots regenerating on ligulate florets inoculated on solid or in liquid MS medium with 8.88 µM∙dm-3 BAP and 2.69 µ M∙dm-3 NAA. The subculture of regenerating ligulate florets from the solid into liquid medium increases the number of regenerating shoots and stimulates their elongation growth, however these shoots are deformed.
pub/13_2_45.pdf Full text available in Adobe Acrobat format:
http://www.hortorumcultus.actapol.net/volume13/issue2/13_2_45.pdf
Keywords: Chrysanthemum × grandiflorum (Ramat.) Kitam., petal, micropropagation, growth regulators

For citation:

MLA Tymoszuk, Alicja, and Małgorzata Zalewska. "In vitro adventitious shoots regeneration from ligulate florets in the aspect of application in chrysanthemum breeding." Acta Sci.Pol. Hortorum Cultus 13.2 (2014): 13.
APA Tymoszuk, A., & Zalewska, M. (2014). In vitro adventitious shoots regeneration from ligulate florets in the aspect of application in chrysanthemum breeding. Acta Sci.Pol. Hortorum Cultus 13 (2), 13.
ISO 690 TYMOSZUK, Alicja, ZALEWSKA, Małgorzata. In vitro adventitious shoots regeneration from ligulate florets in the aspect of application in chrysanthemum breeding. Acta Sci.Pol. Hortorum Cultus, 2014, 13.2: 13.
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Streszczenie w języku polskim:
http://www.hortorumcultus.actapol.net/tom13/zeszyt2/abstrakt-45.html