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Issue 13 (2) 2014 pp. 97–108

Małgorzata Zalewska, Dariusz Kulus

Improvement of Chrysanthemum × grandiflorum (Ramat.) Kitam. encapsulation-dehydration cryopreservation protocol

Abstract: To optimize the protocol, the shoot tips of Chrysanthemum × grandiflorum (Ramat.) Kitam. radiomutants have been cryopreserved using the encapsulation-dehydration technique, following different approaches. In the experiment the influence of abscisic acid – ABA (0; 10; 20 and 30 µM), sucrose concentration (0.09; 0.25 and 0.5 M) and temperature (5ºC or 22ºC) during preculture were tested. Furthermore two dehydration methods, gradual and one-step, were considered. Since the best results were reported with 10 µM ABA + 0.09 M sucrose at room temperature and gradual dehydration, only this combination was selected for the next desiccation-adjustment step. From the time of air desiccation under laminar air-flow applied (0; 1; 2; 3; 4 and 5 hours), 3 hours provided the best results. Due to the growth inhibition on the hormone-free medium, the addition of plant growth regulators during that phase was also tested, confirming the need to apply cytokinins into the medium.
pub/13_2_97.pdf Full text available in Adobe Acrobat format:
http://www.hortorumcultus.actapol.net/volume13/issue2/13_2_97.pdf
Keywords: mutant, ABA, sucrose, preculture, desiccation, regrowth

For citation:

MLA Zalewska, Małgorzata, and Dariusz Kulus. "Improvement of Chrysanthemum × grandiflorum (Ramat.) Kitam. encapsulation-dehydration cryopreservation protocol." Acta Sci.Pol. Hortorum Cultus 13.2 (2014): 13.
APA Zalewska, M., & Kulus, D. (2014). Improvement of Chrysanthemum × grandiflorum (Ramat.) Kitam. encapsulation-dehydration cryopreservation protocol. Acta Sci.Pol. Hortorum Cultus 13 (2), 13.
ISO 690 ZALEWSKA, Małgorzata, KULUS, Dariusz. Improvement of Chrysanthemum × grandiflorum (Ramat.) Kitam. encapsulation-dehydration cryopreservation protocol. Acta Sci.Pol. Hortorum Cultus, 2014, 13.2: 13.
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Streszczenie w języku polskim:
http://www.hortorumcultus.actapol.net/tom13/zeszyt2/abstrakt-97.html