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Polska Bibliografia Naukowa

Issue 13 (5) 2014 pp. 177–185

Henryk Pospieszny, Julia Minicka, Beata Hasiów-Jaroszewska

Cross-protection between different pathotypes of Pepino mosaic virus representing Chilean 2 genotype

Abstract: Viral cross-protection in plants is a phenomenon, where a mild virus isolate can protect plants against damage caused by a severe challenge isolate of the same virus. It has been used on a large scale in cases where no resistant plants are available. We examined differences in cross-protection between pathotypes of Pepino mosaic virus representing Chilean 2 genotype. The potential of a mild PepMV-P22 isolate to protect tomato against more aggressive challenge isolates causing yellowing and necrotic symptoms was established. The challenge isolates were PepMV-P5-IY (yellowing), PepMV-P19 (necrotic) and PepMV-P22 K67E (artificial necrotic mutant of PepMV-P22 which differ from PepMV-P22 only by a point mutation). Efficient cross-protection was obtained using mild PepMV-P22 against PepMV-P5-IY. After a challenge inoculation with PepMV-P19 or PepMV-P22 K67E symptoms severity were significantly reduced in comparison to non-protected plants; however, necrotic symptoms appeared two months after coinfection. The real-time PCR analysis revealed that the level of accumulation of the necrotic isolate in tomato plants was even 5–7 times higher than that of PepMV-P22.
pub/13_5_177.pdf Full text available in Adobe Acrobat format:
http://www.hortorumcultus.actapol.net/volume13/issue5/13_5_177.pdf
Keywords: cross-protection, PepMV, viral variants

For citation:

MLA Pospieszny, Henryk, et al. "Cross-protection between different pathotypes of Pepino mosaic virus representing Chilean 2 genotype." Acta Sci.Pol. Hortorum Cultus 13.5 (2014): 13.
APA Pospieszny, H., Minicka, J., & Hasiów-Jaroszewska, B. (2014). Cross-protection between different pathotypes of Pepino mosaic virus representing Chilean 2 genotype. Acta Sci.Pol. Hortorum Cultus 13 (5), 13.
ISO 690 POSPIESZNY, Henryk, MINICKA, Julia, HASIóW-JAROSZEWSKA, Beata. Cross-protection between different pathotypes of Pepino mosaic virus representing Chilean 2 genotype. Acta Sci.Pol. Hortorum Cultus, 2014, 13.5: 13.
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Streszczenie w języku polskim:
http://www.hortorumcultus.actapol.net/tom13/zeszyt5/abstrakt-177.html