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Polska Bibliografia Naukowa

Issue 14 (6) 2015 pp. 141–150

Eliška Peňázová, Aleš Eichmeier, Jana Čechová, Miroslav Baránek, Robert Pokluda

EVALUATION OF DIFFERENT METHODS OF DNA EXTRACTION FOR DETECTION OF BACTERIUM Xanthomonas campestris pv. Campestris IN CABBAGE LEAVES

Abstract: The bacterium Xanthomonas campestris pathovar campestris (Xcc) as the causal agent of black rot of cruciferous plants causes considerable losses in agricultural yield all over the world. The control of black rot is difficult as well as the determination of Xcc on the basis of morphological parameters or by pathogenicity testing. Ten different possibilities for extraction bacterial DNA followed by PCR detection method were tested to optimize PCR protocol. On the basis of ISTA validated method, three sets of primers UBP 1052F-BACR, DLH 120-125 and ZUP 2309-2310 were used. The results of measured concentration and quality of DNA and efficacy for PCR amplification were compared. Finally, three approaches for DNA extraction within Xanthomonas campestris pv. campestris detection protocol were recommended – commercial kits used for isolation from tissues by Macherey-Nagel and MO BIO and kit intended for microbial cultures by MO BIO.
pub/14_6_141.pdf Full text available in Adobe Acrobat format:
http://www.hortorumcultus.actapol.net/volume14/issue6/14_6_141.pdf
Keywords: black rot, Brassicaceae, specific PCR

For citation:

MLA Peňázová, Eliška, et al. "EVALUATION OF DIFFERENT METHODS OF DNA EXTRACTION FOR DETECTION OF BACTERIUM Xanthomonas campestris pv. Campestris IN CABBAGE LEAVES." Acta Sci.Pol. Hortorum Cultus 14.6 (2015): 14.
APA Peňázová, E., Eichmeier, A., Čechová, J., Baránek, M., & Pokluda, R. (2015). EVALUATION OF DIFFERENT METHODS OF DNA EXTRACTION FOR DETECTION OF BACTERIUM Xanthomonas campestris pv. Campestris IN CABBAGE LEAVES. Acta Sci.Pol. Hortorum Cultus 14 (6), 14.
ISO 690 PEňáZOVá, Eliška, et al. EVALUATION OF DIFFERENT METHODS OF DNA EXTRACTION FOR DETECTION OF BACTERIUM Xanthomonas campestris pv. Campestris IN CABBAGE LEAVES. Acta Sci.Pol. Hortorum Cultus, 2015, 14.6: 14.
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Streszczenie w języku polskim:
http://www.hortorumcultus.actapol.net/tom14/zeszyt6/abstrakt-141.html