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Polska Bibliografia Naukowa

Issue 15 (4) 2016 pp. 17–26

Anna Figas, Magdalena Tomaszewska-Sowa, Anna Sawilska, Anna J. Keutgen

IMPROVEMENT OF in vitro PROPAGATION AND ACCLIMATION OF Helichrysum arenarium L. Moench

Abstract: Explants of apical buds of sandy everlasting Helichrysum arenarium L. Moench sterilized with calcium hypochlorite – Ca(OCl2) were placed onto Murashige-Skoog (MS) growth medium enriched with 1 mg∙dm-3 KIN (kinetin) in two consecutive passages. To optimize the procedure of in vitro micropropagation, the axillary shoots of Helichrysum arenarium L. Moench were transferred during the third passage onto 9 different combinations of MS medium without plant growth regulators (PGRs-free medium), with different concentrations of KIN (1.0, 3.0, 4.0, 5.0 mg∙dm-3) and KIN (1.0, 3.0, 4.0, 5.0 mg∙dm-3) with IAA (indole-3-acetic acid) (0.5 mg dm-3). The highest mean number of shoots (24.7) was observed on MS medium with 5 mg∙dm-3 KIN and 0.5 mg∙dm-3 IAA. The achieved branched shoots were rooted and acclimatized. Rhizogenesis was intensified by the presence of growth hormones: 0.5 mg∙dm-3 IAA or 0.5 mg∙dm-3 IBA (indole-3-butyric acid). At the stage of acclimation of plantlets the application of a water solution of MS salts (25%) for watering the plants increased the efficacy of plant acclimation from 56 to 75%.
pub/15_4_17.pdf Full text available in Adobe Acrobat format:
http://www.hortorumcultus.actapol.net/volume15/issue4/15_4_17.pdf
Keywords: sandy everlasting, phytohormones, rooting, in vivo conditions, medicinal plant

For citation:

MLA Figas, Anna, et al. "IMPROVEMENT OF in vitro PROPAGATION AND ACCLIMATION OF Helichrysum arenarium L. Moench." Acta Sci.Pol. Hortorum Cultus 15.4 (2016): 15.
APA Figas, A., Tomaszewska-Sowa, M., Sawilska, A., & Keutgen, A. J. (2016). IMPROVEMENT OF in vitro PROPAGATION AND ACCLIMATION OF Helichrysum arenarium L. Moench. Acta Sci.Pol. Hortorum Cultus 15 (4), 15.
ISO 690 FIGAS, Anna, et al. IMPROVEMENT OF in vitro PROPAGATION AND ACCLIMATION OF Helichrysum arenarium L. Moench. Acta Sci.Pol. Hortorum Cultus, 2016, 15.4: 15.
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Streszczenie w języku polskim:
http://www.hortorumcultus.actapol.net/tom15/zeszyt4/abstrakt-17.html