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Issue 16 (1) 2017 pp. 85–93

Anna Pindel

REGENERATION CAPACITY OF Asparagus setaceus (Kunth) Jessop ‘PYRAMIDALIS’ IN in vitro CULTURES

Abstract: Plant regeneration through the tissue culture techniques would be a excellent alternative for improving the quality and faster production of Asparagus species. The number of reports on application of biotechnological methods (such as direct organogenesis, indirect organogenesis and somatic embriogenesis) for ornamental asparagus are scarce in comparison to edible asparagus. The aim of this study was to verify the effectiveness of chemical and physical factors on organogenetic response of Asparagus setaceus ‘Pyramidalis’ explants. The results showed that nodes cultured on MS medium supplemented on IAA (1.71 µM) and BA (13.32 µM) gave highest number of shoots per explant (14 – in the fourth passage). Rhizogenesis were achieved on MS medium with IBA (2.45 µM) and ancymidol (2.88 µM). Finally, 90% of rooted shoots (microcuttings) survived. The fastest increase in callus tissue on internodal explants were observed on MS medium with addition of TDZ (9.08 µM) and adenine sulphate (10.86 µM).
pub/16_1_85.pdf Full text available in Adobe Acrobat format:
http://www.hortorumcultus.actapol.net/volume16/issue1/16_1_85.pdf
Keywords: ornamental asparagus, micropropagation, callogenesis

DOI: 10.24326/asphc.2017.1.0

For citation:

MLA Pindel, Anna. "REGENERATION CAPACITY OF Asparagus setaceus (Kunth) Jessop ‘PYRAMIDALIS’ IN in vitro CULTURES." Acta Sci.Pol. Hortorum Cultus 16.1 (2017): 16. DOI: 10.24326/asphc.2017.1.0
APA Pindel, A. (2017). REGENERATION CAPACITY OF Asparagus setaceus (Kunth) Jessop ‘PYRAMIDALIS’ IN in vitro CULTURES. Acta Sci.Pol. Hortorum Cultus 16 (1), 16. DOI: 10.24326/asphc.2017.1.0
ISO 690 PINDEL, Anna. REGENERATION CAPACITY OF Asparagus setaceus (Kunth) Jessop ‘PYRAMIDALIS’ IN in vitro CULTURES. Acta Sci.Pol. Hortorum Cultus, 2017, 16.1: 16. DOI: 10.24326/asphc.2017.1.0
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