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Polska Bibliografia Naukowa

Issue 16 (5) 2017 pp. 97-108

Weronika Haratym, Elżbieta Weryszko-Chmielewska

University of Life Sciences in Lublin, Poland

HISTOCHEMICAL INVESTIGATION OF TRICHOMES AND CHEMICAL COMPOSITION OF ESSENTIAL OIL FROM Euphrasia stricta D. Wolff ex J.F. Lehm. (OROBANCHACEAE)

Abstract:

Micromorphological and anatomical investigations of trichomes on vegetative and generative organs of the drug eyebright (Euphrasia stricta D. Wolff ex J.F. Lehm.) were carried out. Additionally, identification of the main components of the secreted products was conducted. The following types of glandular structures were found: capitate trichomes (i) with a unicellular head, a neck cell, and a 2-celled stalk, (ii) with a unicellular head, a neck cell, and 3-celled stalk, (iii) with a bicellular head and sporadically with a 3- or 4-celled head; moreover, on the surface of corolla unicellular and 2–3-celled clavate trichomes, ribbon-like trichomes, and papillae were present. Using fluorescence microscopy and histochemical tests, various substances such as lipids, phenolic compounds, terpenes containing steroids, terpenoids, sesquiterpenes, tannins, and flavonoids were determined but only in the capitate trichomes. The analysis of the essential oil showed that its content in the dry herb of E. stricta was on average 0.257%. Gas chromatography revealed the presence of 28 compounds.

pub/16_5_97.pdf Full text available in Adobe Acrobat format:
http://www.hortorumcultus.actapol.net/volume16/issue5/16_5_97.pdf
Keywords: chemical compounds, drug eyebright, histochemistry, micromorphology, secretory structure

DOI: 10.24326/asphc.2017.5.10

For citation:

MLA Haratym, Weronika, and Elżbieta Weryszko-Chmielewska. "HISTOCHEMICAL INVESTIGATION OF TRICHOMES AND CHEMICAL COMPOSITION OF ESSENTIAL OIL FROM Euphrasia stricta D. Wolff ex J.F. Lehm. (OROBANCHACEAE)." Acta Sci.Pol. Hortorum Cultus 16.5 (2017): 16. DOI: 10.24326/asphc.2017.5.10
APA Haratym, W., & Weryszko-Chmielewska, E. (2017). HISTOCHEMICAL INVESTIGATION OF TRICHOMES AND CHEMICAL COMPOSITION OF ESSENTIAL OIL FROM Euphrasia stricta D. Wolff ex J.F. Lehm. (OROBANCHACEAE). Acta Sci.Pol. Hortorum Cultus 16 (5), 16. DOI: 10.24326/asphc.2017.5.10
ISO 690 HARATYM, Weronika, WERYSZKO-CHMIELEWSKA, Elżbieta. HISTOCHEMICAL INVESTIGATION OF TRICHOMES AND CHEMICAL COMPOSITION OF ESSENTIAL OIL FROM Euphrasia stricta D. Wolff ex J.F. Lehm. (OROBANCHACEAE). Acta Sci.Pol. Hortorum Cultus, 2017, 16.5: 16. DOI: 10.24326/asphc.2017.5.10
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