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Polska Bibliografia Naukowa

Issue 16 (6) 2017 pp. 149-156

Justyna Lema-Rumińska, Anna Mellem

Laboratory of Biotechnology, Department of Ornamental Plants and Vegetable Crops, UTP University of Science and Technology, Bydgoszcz, Poland

GENETIC DIVERSITY OF CHRYSANTHEMUM PLANTS DERIVED VIA SOMATIC EMBRYOGENESIS USING RAPD MARKERS

Abstract:
The genetic diversity was investigated among two chrysanthemum (<i>Chrysanthemum × grandiflorum</i> Ramat./Kitam.) cultivars ‘Lady Salmon’ and ‘Lady Vitroflora’ and its 15 lines of plants derived from somatic embryos in using ten random amplified polymorphic DNA (RAPD) markers. All primers gave 108 bands with 1218 products from 148.65 to 4391.20 bp in size. The average number of bands per primer was 10.8. Most fragments (54; 50%) were polymorphic, 9 (0.8%) specific and others (45; 49.2%) were monomorphic. Cluster analysis grouped all the cultivars and their lines into two main clusters and two subclusters. Most genetic diversity was characteristic for LS2 lines of plants derived via somatic embryogenesis from cultivar ‘Lady Salmon’. All lines were different from each other and from the original cultivar propagated by meristematic explants. RAPD markers are a helpful tool to detect the genetic diversity of chrysanthemum plants derived via somatic embryogenesis (SE). Our result will provide useful information for production laboratory and for breeding programmes.
pub/16_6_149.pdf Full text available in Adobe Acrobat format:
http://www.hortorumcultus.actapol.net/volume16/issue6/16_6_149.pdf
Keywords: Chrysanthemum × grandiflorum, molecular markers, somatic embryos, genetic distance

DOI: 10.24326/asphc.2017.6.13

For citation:

MLA Lema-Rumińska, Justyna, and Anna Mellem. "GENETIC DIVERSITY OF CHRYSANTHEMUM PLANTS DERIVED VIA SOMATIC EMBRYOGENESIS USING RAPD MARKERS." Acta Sci.Pol. Hortorum Cultus 16.6 (2017): 16. DOI: 10.24326/asphc.2017.6.13
APA Lema-Rumińska, J., & Mellem, A. (2017). GENETIC DIVERSITY OF CHRYSANTHEMUM PLANTS DERIVED VIA SOMATIC EMBRYOGENESIS USING RAPD MARKERS. Acta Sci.Pol. Hortorum Cultus 16 (6), 16. DOI: 10.24326/asphc.2017.6.13
ISO 690 LEMA-RUMIńSKA, Justyna, MELLEM, Anna. GENETIC DIVERSITY OF CHRYSANTHEMUM PLANTS DERIVED VIA SOMATIC EMBRYOGENESIS USING RAPD MARKERS. Acta Sci.Pol. Hortorum Cultus, 2017, 16.6: 16. DOI: 10.24326/asphc.2017.6.13
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