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Polska Bibliografia Naukowa

Issue 16 (6) 2017 pp. 57-66

Agnieszka Szopa, Inga Kwiecień, Halina Ekiert

Department of Pharmaceutical Botany, Jagiellonian University, Collegium Medicum, Kraków, Poland

BIOTRANSFORMATION OF HYDROQUINONE AND 4-HYDROXYBENZOIC ACID IN Schisandra chinensis (CHINESE MAGNOLIA VINE) in vitro CULTURES

Abstract:
Optimization of the process of biotransformation of hydroquinone into its β-D-glucoside – arbutin, was performed in agitated shoot cultures of Schisandra chinensis. The optimisation involved testing various concentrations of the precursor and different ways of administering it. Arbutin was accumulated mainly in the in vitro cultured biomass (85.2–98.6%). By optimizing the process, a 2.26-fold increase in the overall product content was obtained. The highest amount (17.8 mg·g–1 DW) was found after administering 384 mg·l–1 hydroquinone in a dose divided into two portions. An experiment with the biotransformation of 4-hydroxybenzoic acid did not produce arbutin but a mixture of two products of glucosylation of the precursor – hydroxybenzoic acid 4-O-β-glucopyranoside and 4-hydroxybenzoic acid β-glucopyranosyl ester. The identity of all biotransformation products was confirmed by 1H-NMR analysis. The results for the production of arbutin by the biotransformation of hydroquinone are of potential practical importance. On the other hand, the fact of confirming the presence of two glucosylation products has a great cognitive value.
pub/16_6_57.pdf Full text available in Adobe Acrobat format:
http://www.hortorumcultus.actapol.net/volume16/issue6/16_6_57.pdf
Keywords: schizandra, β-glucosylation, arbutin production, agitated shoot cultures, HPLC analysis

DOI: 10.24326/asphc.2017.6.5

For citation:

MLA Szopa, Agnieszka, et al. "BIOTRANSFORMATION OF HYDROQUINONE AND 4-HYDROXYBENZOIC ACID IN Schisandra chinensis (CHINESE MAGNOLIA VINE) in vitro CULTURES." Acta Sci.Pol. Hortorum Cultus 16.6 (2017): 16. DOI: 10.24326/asphc.2017.6.5
APA Szopa, A., Kwiecień, I., & Ekiert, H. (2017). BIOTRANSFORMATION OF HYDROQUINONE AND 4-HYDROXYBENZOIC ACID IN Schisandra chinensis (CHINESE MAGNOLIA VINE) in vitro CULTURES. Acta Sci.Pol. Hortorum Cultus 16 (6), 16. DOI: 10.24326/asphc.2017.6.5
ISO 690 SZOPA, Agnieszka, KWIECIEń, Inga, EKIERT, Halina. BIOTRANSFORMATION OF HYDROQUINONE AND 4-HYDROXYBENZOIC ACID IN Schisandra chinensis (CHINESE MAGNOLIA VINE) in vitro CULTURES. Acta Sci.Pol. Hortorum Cultus, 2017, 16.6: 16. DOI: 10.24326/asphc.2017.6.5
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