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Polska Bibliografia Naukowa

Issue 17 (1) 2018 pp. 139-147

Dariusz Kulus1, Magdalena Serocka1, Anna Mikuła2

1 Faculty of Agriculture and Biotechnology, University of Technology and Life Science in Bydgoszcz, Bernardyńska, Bydgoszcz, Poland
2
Polish Academy of Sciences Botanical Garden – Centre for Biological Diversity Conservation in Powsin, Prawdziwka, Warsaw, Poland

EFFECT OF VARIOUS PRECULTURE AND OSMOTIC DEHYDRATION CONDITIONS ON CRYOPRESERVATION EFFICIENCY AND MORPHOGENETIC RESPONSE OF CHRYSANTHEMUM SHOOT TIPS

Abstract:

The aim of this study was to evaluate the influence of sucrose concentration in the preculture medium, as well as the duration of osmotic dehydration on the efficiency of chrysanthemum ‘Richmond’, ‘Lady Orange’ and ‘Lady Salmon’ cryopreservation by encapsulation-dehydration technique. For all cultivars tested, the best regrowth of cryopreserved shot tips was recorded with 0.25 M sucrose concentration and 10 µM ABA during a two-week preculture, followed by a 4- or 7-day osmotic dehydration. The survival rate ranged from 56.8–58.0% (the Lady group) to 63.6% (‘Richmond’). However, the ability to grow was smaller and reached 18.2–50.7%. It was found that higher sucrose concentration during the preculture slowed the growth of chrysanthemum shoot tips and led to an increased formation of multiple shoots (by activating axillary buds) or deformed adventitious shoots (incapable of further growth). The frequency of tissue hyperhydricity also increased, while the rhizogenesis efficiency decreased when higher sucrose concentration in the preculture medium was applied. The influence of osmotic dehydration duration on the explants morphogenetic response was cultivar-dependent. 

pub/17_1_139.pdf Full text available in Adobe Acrobat format:
http://www.hortorumcultus.actapol.net/volume17/issue1/17_1_139.pdf
Keywords: ABA, chimeras, hyperhydricity, rooting, sucrose, survival evaluation

DOI: 10.24326/asphc.2018.1.13

For citation:

MLA Kulus, Dariusz, et al. "EFFECT OF VARIOUS PRECULTURE AND OSMOTIC DEHYDRATION CONDITIONS ON CRYOPRESERVATION EFFICIENCY AND MORPHOGENETIC RESPONSE OF CHRYSANTHEMUM SHOOT TIPS." Acta Sci.Pol. Hortorum Cultus 17.1 (2018): 17. DOI: 10.24326/asphc.2018.1.13
APA Kulus, D., Serocka, M., & Mikuła, A. (2018). EFFECT OF VARIOUS PRECULTURE AND OSMOTIC DEHYDRATION CONDITIONS ON CRYOPRESERVATION EFFICIENCY AND MORPHOGENETIC RESPONSE OF CHRYSANTHEMUM SHOOT TIPS. Acta Sci.Pol. Hortorum Cultus 17 (1), 17. DOI: 10.24326/asphc.2018.1.13
ISO 690 KULUS, Dariusz, SEROCKA, Magdalena, MIKUłA, Anna. EFFECT OF VARIOUS PRECULTURE AND OSMOTIC DEHYDRATION CONDITIONS ON CRYOPRESERVATION EFFICIENCY AND MORPHOGENETIC RESPONSE OF CHRYSANTHEMUM SHOOT TIPS. Acta Sci.Pol. Hortorum Cultus, 2018, 17.1: 17. DOI: 10.24326/asphc.2018.1.13
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