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Polska Bibliografia Naukowa

Issue 17 (1) 2018 pp. 159-164

Monika Fratrikova1, Miroslav Bauer2,3, Martin Jopcik1, Jana Libantova1

1 Institute of Plant Genetics and Biotechnology, PSBC, SAS, Akademicka, Nitra, Slovak Republic
2
NAFC Research Institute for Animal Production, Nitra, Hlohovska, Lužianky, Slovak Republic
3
Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University, Nábrežie mládeže, Nitra, Slovak Republic

SIMPLE VERIFICATION OF in vitro – GROWN CLONES OF THE GENUS Drosera L. USING ITS MOLECULAR MARKERS

Abstract:

Drosera L. is a genus of carnivorous plants that comprises approximately 250 species, although this number is probably not complete. Some of these taxa exhibit only small differences in morphological traits that can be partly influenced if the taxa are propagated in vitro. Here, we focus on the verification of putative clones of Drosera spathulata Labill., Drosera rotundifolia L. and Drosera binata var. Dichotoma species cultivated in vitro using molecular markers covering the internal transcribed spacer (ITS) region of 45S ribosomal DNA (rDNA). Following the polymerase chain reaction (PCR) amplification of ~360-bp DNA fragments and sequencing, the sequences were aligned with corresponding sequences in the National Center for Biotechnology Information (NCBI) database. In addition, each of tested PCR amplicons had a specific restriction profile that predominantly enables the differentiation of D. rotundifolia and D. spathulata; the shape of the leaves does not have to be a clear morphologically distinguishable trait.

pub/17_1_159.pdf Full text available in Adobe Acrobat format:
http://www.hortorumcultus.actapol.net/volume17/issue1/17_1_159.pdf
Keywords: Drosera binata, Drosera rotundifolia, Drosera spathulata, ITS nucleotide sequences, molecular identi-fication

DOI: 10.24326/asphc.2018.1.15

For citation:

MLA Fratrikova, Monika, et al. "SIMPLE VERIFICATION OF in vitro – GROWN CLONES OF THE GENUS Drosera L. USING ITS MOLECULAR MARKERS." Acta Sci.Pol. Hortorum Cultus 17.1 (2018): 17. DOI: 10.24326/asphc.2018.1.15
APA Fratrikova, M., Bauer, M., Jopcik, M., & Libantova, J. (2018). SIMPLE VERIFICATION OF in vitro – GROWN CLONES OF THE GENUS Drosera L. USING ITS MOLECULAR MARKERS. Acta Sci.Pol. Hortorum Cultus 17 (1), 17. DOI: 10.24326/asphc.2018.1.15
ISO 690 FRATRIKOVA, Monika, et al. SIMPLE VERIFICATION OF in vitro – GROWN CLONES OF THE GENUS Drosera L. USING ITS MOLECULAR MARKERS. Acta Sci.Pol. Hortorum Cultus, 2018, 17.1: 17. DOI: 10.24326/asphc.2018.1.15
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