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Polska Bibliografia Naukowa

Issue 17 (3) 2018 pp. 49-55

Çiğdem Alev Özel

Department of Biology Education, Faculty of Education, Gazi University, Teknikokullar, Ankara, Turkey

EFFICIENT in vitro PLANT REGENERATION FROM CULTURED LEAF AND PETIOLE EXPLANTS OF Isatis constricta Davis

Abstract:

Isatis concstricta Davis (an endemic plant of Turkey) suffers from low propagation rates under natural conditions and is threatened due to fast unplanned urbanisation. The study compared the effects of variants of BA + NAA on shoot regeneration on leaf and petiole explants excised from one week old in vitro regenerated seedlings. MS medium containing 1 mg L–1  BA + 1 mg L–1  NAA induced maximum proliferation on petiole and leaf explants with 13.33 and 12.75 shoots per explant repectively. However, leaf explant induced shoots were sturdy and healthier compared to petiole explant induced shoots. These shoots were rooted on MS medium containing 0.5 mg L–1 IBA and the plants were acclimatized in peat moss and sand (v/v). They grew to flowering under ex vitro conditions. This system of regeneration is advantageous for conventional propagation and the results will help in establishment of a powerful and meaningful micropropagation system for I. constricta.

pub/17_3_49.pdf Full text available in Adobe Acrobat format:
http://www.hortorumcultus.actapol.net/volume17/issue3/17_3_49.pdf
Keywords: direct organogenesis, dye plant, micropropagation, mass proliferation

DOI: 10.24326/asphc.2018.3.5

For citation:

MLA Özel, Çiğdem Alev. "EFFICIENT in vitro PLANT REGENERATION FROM CULTURED LEAF AND PETIOLE EXPLANTS OF Isatis constricta Davis." Acta Sci.Pol. Hortorum Cultus 17.3 (2018): 17. DOI: 10.24326/asphc.2018.3.5
APA Özel, . A. (2018). EFFICIENT in vitro PLANT REGENERATION FROM CULTURED LEAF AND PETIOLE EXPLANTS OF Isatis constricta Davis. Acta Sci.Pol. Hortorum Cultus 17 (3), 17. DOI: 10.24326/asphc.2018.3.5
ISO 690 ÖZEL, Çiğdem Alev. EFFICIENT in vitro PLANT REGENERATION FROM CULTURED LEAF AND PETIOLE EXPLANTS OF Isatis constricta Davis. Acta Sci.Pol. Hortorum Cultus, 2018, 17.3: 17. DOI: 10.24326/asphc.2018.3.5
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