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Issue 5 (1) 2006 pp. 65–70

Danuta Kozak

The effect of growth retardants applied in vitro on the acclimatization and growth of Tibouchina urvilleana Cogn. in vivo

Abstract: Shoot tips of Tibouchina urvilleana Cogn. were cultured 4 weeks in vitro in modified Murashige and Skoog (MS) [1962] medium supplemented with growth retardants: paclobutrazol – 0.1, 0.5, 1.0, 5.0 mg∙dm-3, flurprimidol – 0.1, 1.0, 5.0 mg∙dm-3, chlorocholine chloride (CCC) –5.0, 50.0, 250.0 mg∙dm-3. Explants cultured on the medium without growth substances were used as a control. Rooted microcuttings were transferred to the greenhouse and transplanted into a mixture of 1 peat :  1 perlite, where they were grown for 5 weeks. Plants were then cultivated in a peat substrate during another 5 weeks. Acclimatization of rooted shoots in the greenhouse was affective in 92.5–100%. The survival of plants was lowest when microcuttings were previously cultured on medium with flurprimidol at 5.0 mg∙dm-3. Cultivation of Tibouchina urvilleana shoots in vitro on media with various growth retardants had a significant effect on the further growth of plants ex vitro. Paclobutrazol and flurprimidol at 5.0 mg∙dm-3 inhibited very strong growth of plants after 10 weeks of growth ex vitro.
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Keywords: Tibouchina urvilleana, growth retardants, in vitro, acclimatization, in vivo

For citation:

MLA Kozak, Danuta. "The effect of growth retardants applied in vitro on the acclimatization and growth of Tibouchina urvilleana Cogn. in vivo." Acta Sci.Pol. Hortorum Cultus 5.1 (2006): 5.
APA Kozak, D. (2006). The effect of growth retardants applied in vitro on the acclimatization and growth of Tibouchina urvilleana Cogn. in vivo. Acta Sci.Pol. Hortorum Cultus 5 (1), 5.
ISO 690 KOZAK, Danuta. The effect of growth retardants applied in vitro on the acclimatization and growth of Tibouchina urvilleana Cogn. in vivo. Acta Sci.Pol. Hortorum Cultus, 2006, 5.1: 5.
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Streszczenie w języku polskim:
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